Telomere-binding protein Taz1 controls global replication timing through its localization near late replication origins in fission yeast.

ABSTRACT: In eukaryotes, the replication of chromosome DNA is coordinated by a replication timing program that temporally regulates the firing of individual replication origins. However, the molecular mechanism underlying the program remains elusive. Here, we report that the telomere-binding protein Taz1 plays a crucial role in the control of replication timing in fission yeast. A DNA element located proximal to a late origin in the chromosome arm represses initiation from the origin in early S phase. Systematic deletion and substitution experiments demonstrated that two tandem telomeric repeats are essential for this repression. The telomeric repeats recruit Taz1, a counterpart of human TRF1 and TRF2, to the locus. Genome-wide analysis revealed that Taz1 regulates about half of chromosomal late ori

SUBMITTER: Tazumi A 

PROVIDER: S-EPMC3444731 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature