A PHD12-Snail2 repressive complex epigenetically mediates neural crest epithelial-to-mesenchymal transition.

ABSTRACT: Neural crest cells form within the neural tube and then undergo an epithelial to mesenchymal transition (EMT) to initiate migration to distant locations. The transcriptional repressor Snail2 has been implicated in neural crest EMT via an as of yet unknown mechanism. We report that the adaptor protein PHD12 is highly expressed before neural crest EMT. At cranial levels, loss of PHD12 phenocopies Snail2 knockdown, preventing transcriptional shutdown of the adhesion molecule Cad6b (Cadherin6b), thereby inhibiting neural crest emigration. Although not directly binding to each other, PHD12 and Snail2 both directly interact with Sin3A in vivo, which in turn complexes with histone deacetylase (HDAC). Chromatin immunoprecipitation revealed that PHD12 is recruited to the Cad6b promoter during neura

SUBMITTER: Strobl-Mazzulla PH 

PROVIDER: S-EPMC3444776 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature