Proteome dynamics and early salt stress response of the photosynthetic organism Chlamydomonas reinhardtii.

ABSTRACT:

Background

The cellular proteome and metabolome are underlying dynamic regulation allowing rapid adaptation to changes in the environment. System-wide analysis of these dynamics will provide novel insights into mechanisms of stress adaptation for higher photosynthetic organisms. We applied pulsed-SILAC labeling to a photosynthetic organism for the first time and we established a method to study proteome dynamics in the green alga Chlamydomonas reinhardtii, an emerging model system for plant biology. In addition, we combined the analysis of protein synthesis with metabolic profiling to study the dynamic changes of metabolism and proteome turnover under salt stress conditions.

Results

To study de novo protein synthesis an arginine auxotroph Chlamydomonas strain was cultivated

SUBMITTER: Mastrobuoni G 

PROVIDER: S-EPMC3444938 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature