Rescue the failed half-ZFN by a sensitive mammalian cell-based luciferase reporter system.

ABSTRACT: ZFN technology is a powerful research tool and has been used for genome editing in cells lines, animals and plants. The generation of functional ZFNs for particular targets in mammalian genome is still challenging for an average research group. The modular-assembly method is relatively fast, easy-to-practice but has a high failure rate. Some recent studies suggested that a ZFP with low binding activity might be able to form a working ZFN pair with another binding active half-ZFP. In order to unveil the potential ZFP candidates among those with low binding activities, this paper established a highly sensitive mammalian cell-based transcriptional reporter system to assess the DNA binding activities of ZFPs by inserting multiple copies of ZFN target sequence fragment (TSF) of an interested ge

SUBMITTER: Zhang W 

PROVIDER: S-EPMC3445457 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature