Comparative multi-omics systems analysis of Escherichia coli strains B and K-12.

ABSTRACT:

Background

Elucidation of a genotype-phenotype relationship is critical to understand an organism at the whole-system level. Here, we demonstrate that comparative analyses of multi-omics data combined with a computational modeling approach provide a framework for elucidating the phenotypic characteristics of organisms whose genomes are sequenced.

Results

We present a comprehensive analysis of genome-wide measurements incorporating multifaceted holistic data - genome, transcriptome, proteome, and phenome - to determine the differences between Escherichia coli B and K-12 strains. A genome-scale metabolic network of E. coli B was reconstructed and used to identify genetic bases of the phenotypes unique to B compared with K-12 through in silico complementation testing. This syst

SUBMITTER: Yoon SH 

PROVIDER: S-EPMC3446290 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature