Mediation of Drosophila autosomal dosage effects and compensation by network interactions.

ABSTRACT:

Background

Gene dosage change is a mild perturbation that is a valuable tool for pathway reconstruction in Drosophila. While it is often assumed that reducing gene dose by half leads to two-fold less expression, there is partial autosomal dosage compensation in Drosophila, which may be mediated by feedback or buffering in expression networks.

Results

We profiled expression in engineered flies where gene dose was reduced from two to one. While expression of most one-dose genes was reduced, the gene-specific dose responses were heterogeneous. Expression of two-dose genes that are first-degree neighbors of one-dose genes in novel network models also changed, and the directionality of change depended on the response of one-dose genes.

Conclusions

Our data indicate that ex

SUBMITTER: Malone JH 

PROVIDER: S-EPMC3446302 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature