Paracrine interactions between primary human macrophages and human fibroblasts enhance murine mammary gland humanization in vivo.

ABSTRACT:

Introduction

Macrophages comprise an essential component of the mammary microenvironment necessary for normal gland development. However, there is no viable in vivo model to study their role in normal human breast function. We hypothesized that adding primary human macrophages to the murine mammary gland would enhance and provide a novel approach to examine immune-stromal cell interactions during the humanization process.

Methods

Primary human macrophages, in the presence or absence of ectopic estrogen stimulation, were used to humanize mouse mammary glands. Mechanisms of enhanced humanization were identified by cytokine/chemokine ELISAs, zymography, western analysis, invasion and proliferation assays; results were confirmed with immunohistological analysis.

Results

T

SUBMITTER: Fleming JM 

PROVIDER: S-EPMC3446360 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature