Weak intra-ring allosteric communications of the archaeal chaperonin thermosome revealed by normal mode analysis.

ABSTRACT: Chaperonins are molecular machines that use ATP-driven cycles to assist misfolded substrate proteins to reach the native state. During the functional cycle, these machines adopt distinct nucleotide-dependent conformational states, which reflect large-scale allosteric changes in individual subunits. Distinct allosteric kinetics has been described for the two chaperonin classes. Bacterial (group I) chaperonins, such as GroEL, undergo concerted subunit motions within each ring, whereas archaeal and eukaryotic chaperonins (group II) undergo sequential subunit motions. We study these distinct mechanisms through a comparative normal mode analysis of monomer and double-ring structures of the archaeal chaperonin thermosome and GroEL. We find that thermosome monomers of each type exhibit common low

SUBMITTER: Jayasinghe M 

PROVIDER: S-EPMC3446675 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature