Diversity of active aerobic methanotrophs along depth profiles of arctic and subarctic lake water column and sediments.

ABSTRACT: Methane (CH(4)) emitted from high-latitude lakes accounts for 2-6% of the global atmospheric CH(4) budget. Methanotrophs in lake sediments and water columns mitigate the amount of CH(4) that enters the atmosphere, yet their identity and activity in arctic and subarctic lakes are poorly understood. We used stable isotope probing (SIP), quantitative PCR (Q-PCR), pyrosequencing and enrichment cultures to determine the identity and diversity of active aerobic methanotrophs in the water columns and sediments (0-25 cm) from an arctic tundra lake (Lake Qalluuraq) on the north slope of Alaska and a subarctic taiga lake (Lake Killarney) in Alaska's interior. The water column CH(4) oxidation potential for these shallow (∼2 m deep) lakes was greatest in hypoxic bottom water from the subarctic lake. T

SUBMITTER: He R 

PROVIDER: S-EPMC3446799 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature