Transcriptome tomography for brain analysis in the web-accessible anatomical space.

ABSTRACT: Increased information on the encoded mammalian genome is expected to facilitate an integrated understanding of complex anatomical structure and function based on the knowledge of gene products. Determination of gene expression-anatomy associations is crucial for this understanding. To elicit the association in the three-dimensional (3D) space, we introduce a novel technique for comprehensive mapping of endogenous gene expression into a web-accessible standard space: Transcriptome Tomography. The technique is based on conjugation of sequential tissue-block sectioning, all fractions of which are used for molecular measurements of gene expression densities, and the block- face imaging, which are used for 3D reconstruction of the fractions. To generate a 3D map, tissues are serially sectioned

SUBMITTER: Okamura-Oho Y 

PROVIDER: S-EPMC3446890 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature