Autophagy mediates the delivery of thrombogenic tissue factor to neutrophil extracellular traps in human sepsis.

ABSTRACT:

Background

Sepsis is associated with systemic inflammatory responses and induction of coagulation system. Neutrophil extracellular traps (NETs) constitute an antimicrobial mechanism, recently implicated in thrombosis via platelet entrapment and aggregation.

Methodology/principal findings

In this study, we demonstrate for the first time the localization of thrombogenic tissue factor (TF) in NETs released by neutrophils derived from patients with gram-negative sepsis and normal neutrophils treated with either serum from septic patients or inflammatory mediators involved in the pathogenesis of sepsis. Localization of TF in acidified autophagosomes was observed during this process, as indicated by positive LC3B and LysoTracker staining. Moreover, phosphatidylinositol 3-kinase in

SUBMITTER: Kambas K 

PROVIDER: S-EPMC3446899 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature