High resolution melt analysis (HRMA); a viable alternative to agarose gel electrophoresis for mouse genotyping.

ABSTRACT: Most mouse genetics laboratories maintain mouse strains that require genotyping in order to identify the genetically modified animals. The plethora of mutagenesis strategies and publicly available mouse alleles means that any one laboratory may maintain alleles with random or targeted insertions of orthologous or unrelated sequences as well as random or targeted deletions and point mutants. Many experiments require that different strains be cross bred conferring the need to genotype progeny at more than one locus. In contrast to the range of new technologies for mouse mutagenesis, genotyping methods have remained relatively static with alleles typically discriminated by agarose gel electrophoresis of PCR products. This requires a large amount of researcher time. Additionally it is suscepti

SUBMITTER: Thomsen N 

PROVIDER: S-EPMC3446988 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature