A receptor-targeted fluorescent radiopharmaceutical for multireporter sentinel lymph node imaging.

ABSTRACT:

Purpose

To determine the imaging and receptor-binding properties of a multireporter probe designed for sentinel lymph node (SLN) mapping via nuclear and fluorescence detection.

Materials and methods

The animal experiments were approved by the institutional animal care and use committee. A multireporter probe was synthesized by covalently attaching cyanine 7 (Cy7), a near-infrared cyanine dye, to tilmanocept, a radiopharmaceutical that binds to a receptor specific to recticuloendothelial cells. In vitro binding assays of technetium 99m (99mTc)-labeled Cy7 tilmanocept were conducted at 4°C by using receptor-bearing macrophages. Optical SLN imaging after foot pad administration was performed by using two molar doses of Cy7 tilmanocept. Six mice were injected with 0.11 nmol of 9

SUBMITTER: Emerson DK 

PROVIDER: S-EPMC3447170 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature