Alternative Splicing of a Novel Inducible Exon Diversifies the CASK Guanylate Kinase Domain.

ABSTRACT: Alternative pre-mRNA splicing has a major impact on cellular functions and development with the potential to fine-tune cellular localization, posttranslational modification, interaction properties, and expression levels of cognate proteins. The plasticity of regulation sets the stage for cells to adjust the relative levels of spliced mRNA isoforms in response to stress or stimulation. As part of an exon profiling analysis of mouse cortical neurons stimulated with high KCl to induce membrane depolarization, we detected a previously unrecognized exon (E24a) of the CASK gene, which encodes for a conserved peptide insertion in the guanylate kinase interaction domain. Comparative sequence analysis shows that E24a appeared selectively in mammalian CASK genes as part of a >3,000 base pair intron

SUBMITTER: Dembowski JA 

PROVIDER: S-EPMC3447378 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature