RNA sequencing revealed novel actors of the acquisition of drug resistance in Candida albicans.

ABSTRACT:

Background

Drug susceptible clinical isolates of Candida albicans frequently become highly tolerant to drugs during chemotherapy, with dreadful consequences to patient health. We used RNA sequencing (RNA-seq) to analyze the transcriptomes of a CDR (Candida Drug Resistance) strain and its isogenic drug sensitive counterpart.

Results

RNA-seq unveiled differential expression of 228 genes including a) genes previously identified as involved in CDR, b) genes not previously associated to the CDR phenotype, and c) novel transcripts whose function as a gene is uncharacterized. In particular, we show for the first time that CDR acquisition is correlated with an overexpression of the transcription factor encoding gene CZF1. CZF1 null mutants were susceptible to many drugs, independent

SUBMITTER: Dhamgaye S 

PROVIDER: S-EPMC3447688 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature