Genome-wide prediction and validation of sigma70 promoters in Lactobacillus plantarum WCFS1.

ABSTRACT:

Background

In prokaryotes, sigma factors are essential for directing the transcription machinery towards promoters. Various sigma factors have been described that recognize, and bind to specific DNA sequence motifs in promoter sequences. The canonical sigma factor σ(70) is commonly involved in transcription of the cell's housekeeping genes, which is mediated by the conserved σ(70) promoter sequence motifs. In this study the σ(70)-promoter sequences in Lactobacillus plantarum WCFS1 were predicted using a genome-wide analysis. The accuracy of the transcriptionally-active part of this promoter prediction was subsequently evaluated by correlating locations of predicted promoters with transcription start sites inferred from the 5'-ends of transcripts detected by high-resolution tiling a

SUBMITTER: Todt TJ 

PROVIDER: S-EPMC3447810 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature