Modelling neuroinflammation in vitro: a tool to test the potential neuroprotective effect of anti-inflammatory agents.

ABSTRACT: Neuron-microglia co-cultures treated with pro-inflammatory agents are a useful tool to study neuroinflammation in vitro, where to test the potential neuroprotective effect of anti-inflammatory compounds. However, a great diversity of experimental conditions can be found in the literature, making difficult to select the working conditions when considering this approach for the first time. We compared the use of neuron-primary microglia and neuron-BV2 cells (a microglial cell line) co-cultures, using different neuron:microglia ratios, treatments and time post-treatment to induce glial activation and derived neurotoxicity. We show that each model requires different experimental conditions, but that both neuron-BV2 and neuron-primary microglia LPS/IFN-γ-treated co-cultures are good to study th

SUBMITTER: Gresa-Arribas N 

PROVIDER: S-EPMC3447933 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature