A facile method to synthesize histones with posttranslational modification mimics.

ABSTRACT: Using an evolved pyrrolysyl-tRNA synthetase-tRNA(Pyl) pair, a Se-alkylselenocysteine was genetically incorporated into histone H3 with a high protein expression yield. Quantitative oxidative elimination of Se-alkylselenocysteine followed by Michael addition reactions with various thiol nucleophiles generated biologically active mimics of H3 with posttranslational modifications including lysine methylation, lysine acetylation, and serine phosphorylation.

SUBMITTER: Wang ZU 

PROVIDER: S-EPMC3448024 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature