Using flow cytometry to estimate pollen DNA content: improved methodology and applications.

ABSTRACT:

Background and aims

Flow cytometry has been used to measure nuclear DNA content in pollen, mostly to understand pollen development and detect unreduced gametes. Published data have not always met the high-quality standards required for some applications, in part due to difficulties inherent in the extraction of nuclei. Here we describe a simple and relatively novel method for extracting pollen nuclei, involving the bursting of pollen through a nylon mesh, compare it with other methods and demonstrate its broad applicability and utility.

Methods

The method was tested across 80 species, 64 genera and 33 families, and the data were evaluated using established criteria for estimating genome size and analysing cell cycle. Filter bursting was directly compared with chopping in fiv

SUBMITTER: Kron P 

PROVIDER: S-EPMC3448423 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature