Spinophilin as a novel regulator of M3 muscarinic receptor-mediated insulin release in vitro and in vivo.

ABSTRACT: Spinophilin (SPL), a multidomain scaffolding protein known to modulate the activity of different G-protein-coupled receptors, regulates various central nervous system (CNS) functions. However, little is known about the role of SPL expressed in peripheral cell types including pancreatic β cells. In this study, we examined the ability of SPL to modulate the activity of β-cell M(3) muscarinic acetylcholine receptors (M3Rs), which play an important role in facilitating insulin release and maintaining normal blood glucose levels. We demonstrated, by using both in vitro and in vivo approaches (mouse insulinoma cells and SPL-deficient mice), that SPL is a potent negative regulator of M3R-mediated signaling and insulin release. Additional biochemical and biophysical studies, including the use of b

SUBMITTER: Ruiz de Azua I 

PROVIDER: S-EPMC3448777 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature