Protective effects of peroxisome proliferator-activated receptor agonists on human podocytes: proposed mechanisms of action.

ABSTRACT:

Background and purpose

Peroxisome proliferator-activated receptor (PPAR) agonists exert anti-albuminuric effects. However, the nephroprotective effects of these drugs remain to be fully understood. We have investigated whether gemfibrozil, GW0742 and pioglitazone protect human podocytes against nutrient deprivation (ND)-induced cell death and the role of mitochondrial biogenesis as a cytoprotective process.

Experimental approach

Immortalized human podocytes were pre-treated with the PPAR agonists and exposed to ND (5 h) under normoxia, hypoxia or in the presence of pyruvate. Cell death was measured at the end of the ND and of the recovery phase (24 h). Mitochondrial mass, cytochrome c oxidase (COX) subunits 1 and 4 were measured as markers of mitochondrial cell content, whil

SUBMITTER: Miglio G 

PROVIDER: S-EPMC3449267 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature