Growth control of hybridoma cells with an artificially induced EpoR-gp130 heterodimer.

ABSTRACT: IL-6 has been known to modulate the growth of many hybridoma cells and also promote resultant antibody productivity. However, IL-6 is so expensive that the use of IL-6-dependent hybridomas for industrial antibody production is not practical. In this study, we aimed at designing antibody/gp130 and antibody/EpoR chimeras which could tightly control cell growth in response to more affordable cognate antigen. Retroviral vectors encoding V(H) or V(L) region of anti-hen egg lysozyme (HEL) antibody HyHEL-10 tethered to a pair of extracellular D2/transmembrane domains of erythropoietin receptor (EpoR) and cytoplasmic domains of either EpoR or gp130, were constructed, and a homodimeric or a heterodimeric pair of chimeric receptor combinations (V(H)-gp130 and V(L)-gp130 or V(H)-gp130 and V(L)-EpoR)

SUBMITTER: Kawahara M 

PROVIDER: S-EPMC3449410 | biostudies-literature | 2006 Nov

REPOSITORIES: biostudies-literature