Alteration in expression of the rat mitochondrial ATPase 6 gene during Pneumocystis carinii infection.

ABSTRACT:

Background

Pneumocystis carinii causes pneumonia in immunocompromised patients with a high morbidity and mortality rate, but the interaction between this organism and the host cell is not well understood. The purpose of this research was to study the response of host cells to P. carinii infection on a molecular level.

Results

The technique of mRNA differential display was used to detect genes whose expression may be affected by P. carinii infection. The nucleotide sequence of one differentially displayed DNA fragment was found to be identical to that of the rat mitochondrial ATPase 6 gene, which is a subunit of the F0F1-ATP synthase complex. A four-fold increase in expression of this gene was verified by Northern blot analysis of total RNA extracted from P. carinii-infected

SUBMITTER: Asnicar MA 

PROVIDER: S-EPMC34520 | biostudies-literature | 2001

REPOSITORIES: biostudies-literature