The Relationship between fenestrations, sieve plates and rafts in liver sinusoidal endothelial cells.

ABSTRACT: Fenestrations are transcellular pores in endothelial cells that facilitate transfer of substrates between blood and the extravascular compartment. In order to understand the regulation and formation of fenestrations, the relationship between membrane rafts and fenestrations was investigated in liver sinusoidal endothelial cells where fenestrations are grouped into sieve plates. Three dimensional structured illumination microscopy, scanning electron microscopy, internal reflectance fluorescence microscopy and two-photon fluorescence microscopy were used to study liver sinusoidal endothelial cells isolated from mice. There was an inverse distribution between sieve plates and membrane rafts visualized by structured illumination microscopy and the fluorescent raft stain, Bodipy FL C5 gangliosi

SUBMITTER: Svistounov D 

PROVIDER: S-EPMC3454341 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature