Isolation of DNA aptamers using micro free flow electrophoresis.

ABSTRACT: A micro free flow electrophoresis (μFFE) device was used to select DNA aptamers for human immunoglobulin E (IgE). The continuous nature of μFFE allowed 1.8 × 10(14) sequences to be introduced over a period of 30 min, a 300-fold improvement in library size over capillary electrophoresis based selections (CE-SELEX). Four rounds of selection were performed within four days. Aptamers with low nM dissociation constants for IgE were identified after a single round of μFFE selection.

SUBMITTER: Jing M 

PROVIDER: S-EPMC3454500 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature