Analysis of meiotic segregation patterns and interchromosomal effects in sperm from six males with Robertsonian translocations.

ABSTRACT:

Purpose

To provide more genetic information about meiotic segregation behavior and the possibility of interchromosomal effects (ICE) in spermatozoa from carriers of Robertsonian (Rob) translocations.

Materials and methods

Meiotic segregation behavior in spermatozoa from six carriers of Rob translocations, four t(13;14), one t(14;22) and one t(13;21), was investigated by dual fluorescence in-situ hybridization (FISH). Aneuploidy for chromosomes 18, X and Y was studied by triple FISH.

Results

The rate of normal/balanced spermatozoa resulting from alternate segregation ranged from 78.14 to 86.88%. The frequency of unbalanced spermatozoa resulting from adjacent segregation varied between 11.70 and 19.53%. The higher frequencies of aneuploidy for sex chromosome were observ

SUBMITTER: Chen Y 

PROVIDER: S-EPMC3454947 | biostudies-literature | 2007 Sep

REPOSITORIES: biostudies-literature