Reverse genetics recovery of Lujo virus and role of virus RNA secondary structures in efficient virus growth.

ABSTRACT: Arenaviruses are rodent-borne viruses with a bisegmented RNA genome. A genetically unique arenavirus, Lujo virus, was recently discovered as the causal agent of a nosocomial outbreak of acute febrile illness with hemorrhagic manifestations in Zambia and South Africa. The outbreak had a case fatality rate of 80%. A reverse genetics system to rescue infectious Lujo virus from cDNA was established to investigate the biological properties of this virus. Sequencing the genomic termini showed unique nucleotides at the 3' terminus of the S segment promoter element. While developing this system, we discovered that reconstructing infectious Lujo virus using the previously reported L segment intergenic region (IGR), comprising the arenaviral transcription termination signal, yielded an attenuated Lu

SUBMITTER: Bergeron E 

PROVIDER: S-EPMC3457263 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature