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Transposon mutagenesis of Planctomyces limnophilus and analysis of a pckA mutant.

ABSTRACT: A Planctomyces limnophilus mutant generated using the EZ-Tn5 transposome was found to possess an insertion within pckA, encoding phosphoenolpyruvate carboxykinase. Disruption of pckA expression and elimination of enzymatic activity resulted in poor growth in glucose-free medium, demonstrating a gluconeogenic role for pckA in P. limnophilus.

SUBMITTER: Schreier HJ 

PROVIDER: S-EPMC3457482 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature

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Transposon mutagenesis of Planctomyces limnophilus and analysis of a pckA mutant.

Schreier Harold J HJ   Dejtisakdi Wipawee W   Escalante Jose Ortega JO   Brailo Marina M  

Applied and environmental microbiology 20120713 19

A Planctomyces limnophilus mutant generated using the EZ-Tn5 transposome was found to possess an insertion within pckA, encoding phosphoenolpyruvate carboxykinase. Disruption of pckA expression and elimination of enzymatic activity resulted in poor growth in glucose-free medium, demonstrating a gluconeogenic role for pckA in P. limnophilus. ...[more]

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