Identification of critical host mitochondrion-associated genes during Ehrlichia chaffeensis infections.

ABSTRACT: Ehrlichia chaffeensis is an obligate intracellular bacterium that causes human monocytic ehrlichiosis (HME). To determine what host components are important for bacterial replication, we performed microarray analysis on Drosophila melanogaster S2 cells by comparing host gene transcript levels between permissive and nonpermissive conditions for E. chaffeensis growth. Five-hundred twenty-seven genes had increased transcript levels unique to permissive growth conditions 24 h postinfection. We screened adult flies that were mutants for several of the "permissive" genes for the ability to support Ehrlichia replication. Three additional D. melanogaster fly lines with putative mutations in pyrimidine metabolism were also tested. Ten fly lines carrying mutations in the genes CG6479, separation anx

SUBMITTER: Von Ohlen T 

PROVIDER: S-EPMC3457586 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature