Protocol: High-throughput and quantitative assays of auxin and auxin precursors from minute tissue samples.

ABSTRACT:

Background

The plant hormone auxin, indole-3-acetic acid (IAA), plays important roles in plant growth and development. The signaling response to IAA is largely dependent on the local concentration of IAA, and this concentration is regulated by multiple mechanisms in plants. Therefore, the precise quantification of local IAA concentration provides insights into the regulation of IAA and its biological roles. Meanwhile, pathways and genes involved in IAA biosynthesis are not fully understood, so it is necessary to analyze the production of IAA at the metabolite level for unbiased studies of IAA biosynthesis.

Results

We have developed high-throughput methods to quantify plant endogenous IAA and its biosynthetic precursors including indole, tryptophan, indole-3-pyruvic acid (IPy

SUBMITTER: Liu X 

PROVIDER: S-EPMC3457856 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature