Potential role of multiple carbon fixation pathways during lipid accumulation in Phaeodactylum tricornutum.

ABSTRACT:

Background

Phaeodactylum tricornutum is a unicellular diatom in the class Bacillariophyceae. The full genome has been sequenced (<30 Mb), and approximately 20 to 30% triacylglyceride (TAG) accumulation on a dry cell basis has been reported under different growth conditions. To elucidate P. tricornutum gene expression profiles during nutrient-deprivation and lipid-accumulation, cell cultures were grown with a nitrate to phosphate ratio of 20:1 (N:P) and whole-genome transcripts were monitored over time via RNA-sequence determination.

Results

The specific Nile Red (NR) fluorescence (NR fluorescence per cell) increased over time; however, the increase in NR fluorescence was initiated before external nitrate was completely exhausted. Exogenous phosphate was depleted before nitra

SUBMITTER: Valenzuela J 

PROVIDER: S-EPMC3457861 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature