Evaluation of human antibody responses to keyhole limpet hemocyanin on a carbohydrate microarray.

ABSTRACT:

Purpose

Keyhole limpet hemocyanin (KLH) is used as a vaccine adjuvant, as a carrier protein for small haptens, and as a treatment for bladder cancer. Immunization with KLH produces antibodies to tumor-associated carbohydrate antigens (TACAs) in animals, and these antibodies have been postulated as the basis of efficacy for bladder cancer treatment. The purpose of this study was to evaluate antibody responses to KLH in humans.

Experimental design

A carbohydrate microarray was used to profile antibody responses in 14 individuals immunized with KLH plus alum adjuvant.

Results

Eight out of fourteen individuals produced antibodies to at least one TACA. Increases to Lewis X, Lewis Y, GA1di, GM3, and sialyl Lewis A were observed in certain individuals, but, in general, antib

SUBMITTER: Oyelaran O 

PROVIDER: S-EPMC3457918 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature