PCR primers for metazoan nuclear 18S and 28S ribosomal DNA sequences.

ABSTRACT:

Background

Metagenetic analyses, which amplify and sequence target marker DNA regions from environmental samples, are increasingly employed to assess the biodiversity of communities of small organisms. Using this approach, our understanding of microbial diversity has expanded greatly. In contrast, only a few studies using this approach to characterize metazoan diversity have been reported, despite the fact that many metazoan species are small and difficult to identify or are undescribed. One of the reasons for this discrepancy is the availability of universal primers for the target taxa. In microbial studies, analysis of the 16S ribosomal DNA is standard. In contrast, the best gene for metazoan metagenetics is less clear. In the present study, we have designed primers that amplify

SUBMITTER: Machida RJ 

PROVIDER: S-EPMC3458000 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature