Discovery, optimization and validation of an optimal DNA-binding sequence for the Six1 homeodomain transcription factor.

ABSTRACT: The Six1 transcription factor is a homeodomain protein involved in controlling gene expression during embryonic development. Six1 establishes gene expression profiles that enable skeletal myogenesis and nephrogenesis, among others. While several homeodomain factors have been extensively characterized with regards to their DNA-binding properties, relatively little is known of the properties of Six1. We have used the genomic binding profile of Six1 during the myogenic differentiation of myoblasts to obtain a better understanding of its preferences for recognizing certain DNA sequences. DNA sequence analyses on our genomic binding dataset, combined with biochemical characterization using binding assays, reveal that Six1 has a much broader DNA-binding sequence spectrum than had been previously

SUBMITTER: Liu Y 

PROVIDER: S-EPMC3458543 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature