Characterization of aldehyde dehydrogenase isozymes in ovarian cancer tissues and sphere cultures.

ABSTRACT:

Background

Aldehyde dehydrogenases belong to a superfamily of detoxifying enzymes that protect cells from carcinogenic aldehydes. Of the superfamily, ALDH1A1 has gained most attention because current studies have shown that its expression is associated with human cancer stem cells. However, ALDH1A1 is only one of the 19 human ALDH subfamilies currently known. The purpose of the present study was to determine if the expression and activities of other major ALDH isozymes are associated with human ovarian cancer and ovarian cancer sphere cultures.

Methods

Immunohistochemistry was used to delineate ALDH isozyme localization in clinical ovarian tissues. Western Blot analyses were performed on lysates prepared from cancer cell lines and ovarian cancer spheres to confirm the immuno

SUBMITTER: Saw YT 

PROVIDER: S-EPMC3458927 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature