DNA damage defines sites of recurrent chromosomal translocations in B lymphocytes.

ABSTRACT: Recurrent chromosomal translocations underlie both haematopoietic and solid tumours. Their origin has been ascribed to selection of random rearrangements, targeted DNA damage, or frequent nuclear interactions between translocation partners; however, the relative contribution of each of these elements has not been measured directly or on a large scale. Here we examine the role of nuclear architecture and frequency of DNA damage in the genesis of chromosomal translocations by measuring these parameters simultaneously in cultured mouse B lymphocytes. In the absence of recurrent DNA damage, translocations between Igh or Myc and all other genes are directly related to their contact frequency. Conversely, translocations associated with recurrent site-directed DNA damage are proportional to the r

SUBMITTER: Hakim O 

PROVIDER: S-EPMC3459314 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature