Computational identification and experimental validation of microRNAs binding to the Alzheimer-related gene ADAM10.

ABSTRACT:

Background

MicroRNAs (miRNAs) are post-transcriptional regulators involved in numerous biological processes including the pathogenesis of Alzheimer's disease (AD). A key gene of AD, ADAM10, controls the proteolytic processing of APP and the formation of the amyloid plaques and is known to be regulated by miRNA in hepatic cancer cell lines. To predict miRNAs regulating ADAM10 expression concerning AD, we developed a computational approach.

Methods

MiRNA binding sites in the human ADAM10 3' untranslated region were predicted using the RNA22, RNAhybrid and miRanda programs and ranked by specific selection criteria with respect to AD such as differential regulation in AD patients and tissue-specific expression. Furthermore, target genes of miR-103, miR-107 and miR-1306 were deri

SUBMITTER: Augustin R 

PROVIDER: S-EPMC3459808 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature