Dynactin's pointed-end complex is a cargo-targeting module.

ABSTRACT: Dynactin is an essential part of the cytoplasmic dynein motor that enhances motor processivity and serves as an adaptor that allows dynein to bind cargoes. Much is known about dynactin's interaction with dynein and microtubules, but how it associates with its diverse complement of subcellular binding partners remains mysterious. It has been suggested that cargo specification involves a group of subunits referred to as the "pointed-end complex." We used chemical cross-linking, RNA interference, and protein overexpression to characterize interactions within the pointed-end complex and explore how it contributes to dynactin's interactions with endomembranes. The Arp11 subunit, which caps one end of dynactin's Arp1 filament, and p62, which binds Arp11 and Arp1, are necessary for dynactin stabi

SUBMITTER: Yeh TY 

PROVIDER: S-EPMC3459859 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature