Complex chromosomal rearrangements mediated by break-induced replication involve structure-selective endonucleases.

ABSTRACT: DNA double-strand break (DSB) repair occurring in repeated DNA sequences often leads to the generation of chromosomal rearrangements. Homologous recombination normally ensures a faithful repair of DSBs through a mechanism that transfers the genetic information of an intact donor template to the broken molecule. When only one DSB end shares homology to the donor template, conventional gene conversion fails to occur and repair can be channeled to a recombination-dependent replication pathway termed break-induced replication (BIR), which is prone to produce chromosome non-reciprocal translocations (NRTs), a classical feature of numerous human cancers. Using a newly designed substrate for the analysis of DSB-induced chromosomal translocations, we show that Mus81 and Yen1 structure-selective en

SUBMITTER: Pardo B 

PROVIDER: S-EPMC3459980 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature