SWI/SNF-like chromatin remodeling factor Fun30 supports point centromere function in S. cerevisiae.

ABSTRACT: Budding yeast centromeres are sequence-defined point centromeres and are, unlike in many other organisms, not embedded in heterochromatin. Here we show that Fun30, a poorly understood SWI/SNF-like chromatin remodeling factor conserved in humans, promotes point centromere function through the formation of correct chromatin architecture at centromeres. Our determination of the genome-wide binding and nucleosome positioning properties of Fun30 shows that this enzyme is consistently enriched over centromeres and that a majority of CENs show Fun30-dependent changes in flanking nucleosome position and/or CEN core micrococcal nuclease accessibility. Fun30 deletion leads to defects in histone variant Htz1 occupancy genome-wide, including at and around most centromeres. FUN30 genetically interacts

SUBMITTER: Durand-Dubief M 

PROVIDER: S-EPMC3459985 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature