Selection and validation of reference genes for quantitative RT-PCR expression studies of the non-model crop Musa.

ABSTRACT: Gene expression analysis by reverse transcriptase real-time or quantitative polymerase chain reaction (RT-qPCR) is becoming widely used for non-model plant species. Given the high sensitivity of this method, normalization using multiple housekeeping or reference genes is critical, and careful selection of these reference genes is one of the most important steps to obtain reliable results. In this study, reference genes commonly used for other plant species were investigated to identify genes displaying highly uniform expression patterns in different varieties, tissues, developmental stages, fungal infection, and osmotic stress conditions for the non-model crop Musa (banana and plantains). The expression stability of six candidate reference genes was tested on six different sample sets, and

SUBMITTER: Podevin N 

PROVIDER: S-EPMC3460175 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature