Nutritional control of mRNA isoform expression during developmental arrest and recovery in C. elegans.

ABSTRACT: Nutrient availability profoundly influences gene expression. Many animal genes encode multiple transcript isoforms, yet the effect of nutrient availability on transcript isoform expression has not been studied in genome-wide fashion. When Caenorhabditis elegans larvae hatch without food, they arrest development in the first larval stage (L1 arrest). Starved larvae can survive L1 arrest for weeks, but growth and post-embryonic development are rapidly initiated in response to feeding. We used RNA-seq to characterize the transcriptome during L1 arrest and over time after feeding. Twenty-seven percent of detectable protein-coding genes were differentially expressed during recovery from L1 arrest, with the majority of changes initiating within the first hour, demonstrating widespread, acute eff

SUBMITTER: Maxwell CS 

PROVIDER: S-EPMC3460187 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature