Gene structure in the sea urchin Strongylocentrotus purpuratus based on transcriptome analysis.

ABSTRACT: A comprehensive transcriptome analysis has been performed on protein-coding RNAs of Strongylocentrotus purpuratus, including 10 different embryonic stages, six feeding larval and metamorphosed juvenile stages, and six adult tissues. In this study, we pooled the transcriptomes from all of these sources and focused on the insights they provide for gene structure in the genome of this recently sequenced model system. The genome had initially been annotated by use of computational gene model prediction algorithms. A large fraction of these predicted genes were recovered in the transcriptome when the reads were mapped to the genome and appropriately filtered and analyzed. However, in a manually curated subset, we discovered that more than half the computational gene model predictions were imper

SUBMITTER: Tu Q 

PROVIDER: S-EPMC3460201 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature