Genomic and Physiological Analysis of Carbon Storage in the Verrucomicrobial Methanotroph "Ca. Methylacidiphilum Fumariolicum" SolV.

ABSTRACT: "Candidatus Methylacidiphilum fumariolicum" SolV is a verrucomicrobial methanotroph that can grow in extremely acidic environments at high temperature. Strain SolV fixes carbon dioxide (CO(2)) via the Calvin-Benson-Bassham cycle with methane as energy source, a trait so far very unusual in methanotrophs. In this study, the ability of "Ca. M. fumariolicum" to store carbon was explored by genome analysis, physiological studies, and electron microscopy. When cell cultures were depleted for nitrogen, glycogen storage was clearly observed in cytoplasmic storage vesicles by electron microscopy. After cessation of growth, the dry weight kept increasing and the bacteria were filled up almost entirely by glycogen. This was confirmed by biochemical analysis, which showed that glycogen accumulated to

SUBMITTER: Khadem AF 

PROVIDER: S-EPMC3460235 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature