High immunogenicity of the human leukocyte antigen peptidomes of melanoma tumor cells.

ABSTRACT: Human leukocyte antigens (HLA) bind peptides generated by limited proteolysis in cells and present them at the cell surfaces for recognition by T cells. Through this antigen presentation function they control the specificity of T cell responses and thereby adaptive immune responses. Knowledge of HLA-bound peptides is thus key to understanding adaptive immunity and to the development of vaccines and other specific immune intervention strategies. To gain insight into the antigenicity of melanomas, peptides were extracted from HLA isolated from the tumor cells, separated by two-dimensional HPLC, and sequenced by mass spectrometry. The spectra were analyzed by database-dependent MASCOT searches and database-independent de novo sequencing and, where required, confirmed with synthetic peptides,

SUBMITTER: Jarmalavicius S 

PROVIDER: S-EPMC3460442 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature