Molecular characterisation of endogenous Vangl2/Vangl1 heteromeric protein complexes.

ABSTRACT:

Background

Mutations in the Planar Cell Polarity (PCP) core gene Vangl2 cause the most severe neural tube defects (NTD) in mice and humans. Genetic studies show that the Vangl2 gene genetically interacts with a close homologue Vangl1. How precisely Vangl2 and Vangl1 proteins interact and crosstalk has remained a difficult issue to address, with the main obstacle being the accurate discrimination of the two proteins, which share close sequence homology. Experimental evidence previously presented has been sparse and addressed with ectopically expressed proteins or with antibodies unable to biochemically discriminate Vangl1 from Vangl2, therefore giving rise to unclear results.

Methodology and main findings

A highly specific monoclonal anti-Vangl2 antibody was generated and rig

SUBMITTER: Belotti E 

PROVIDER: S-EPMC3460870 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature