Hypoxic conditions induce a cancer-like phenotype in human breast epithelial cells.

ABSTRACT:

Introduction

Solid tumors are less oxygenated than their tissue of origin. Low intra-tumor oxygen levels are associated with worse outcome, increased metastatic potential and immature phenotype in breast cancer. We have reported that tumor hypoxia correlates to low differentiation status in breast cancer. Less is known about effects of hypoxia on non-malignant cells. Here we address whether hypoxia influences the differentiation stage of non-malignant breast epithelial cells and potentially have bearing on early stages of tumorigenesis.

Methods

Normal human primary breast epithelial cells and immortalized non-malignant mammary epithelial MCF-10A cells were grown in a three-dimensional overlay culture on laminin-rich extracellular matrix for up to 21 days at normoxic or hypox

SUBMITTER: Vaapil M 

PROVIDER: S-EPMC3460905 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature