Retinoic acid signalling is activated in the postischemic heart and may influence remodelling.

ABSTRACT:

Background

All-trans retinoic acid (atRA), an active derivative of vitamin A, regulates cell differentiation, proliferation and cardiac morphogenesis via transcriptional activation of retinoic acid receptors (RARs) acting on retinoic acid response elements (RARE). We hypothesized that the retinoic acid (RA) signalling pathway is activated in myocardial ischemia and postischemic remodelling.

Methods and findings

Myocardial infarction was induced through ligating the left coronary artery in mice. In vivo cardiac activation of the RARs was measured by imaging RARE-luciferase reporter mice, and analysing expression of RAR target genes and proteins by real time RT-PCR and western blot. Endogenous retinoids in postinfarcted hearts were analysed by triple-stage liquid chromatograph

SUBMITTER: Bilbija D 

PROVIDER: S-EPMC3460971 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature