Human cardiac progenitor cells engineered with Pim-I kinase enhance myocardial repair.

ABSTRACT:

Objectives

The goal of this study was to demonstrate the enhancement of human cardiac progenitor cell (hCPC) reparative and regenerative potential by genetic modification for the treatment of myocardial infarction.

Background

Regenerative potential of stem cells to repair acute infarction is limited. Improved hCPC survival, proliferation, and differentiation into functional myocardium will increase efficacy and advance translational implementation of cardiac regeneration.

Methods

hCPCs isolated from the myocardium of heart failure patients undergoing left ventricular assist device implantation were engineered to express green fluorescent protein (hCPCe) or Pim-1-GFP (hCPCeP). Functional tests of hCPC regenerative potential were performed with immunocompromised mice by

SUBMITTER: Mohsin S 

PROVIDER: S-EPMC3461098 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature