Comparative analysis of different approaches to measure treatment response in acute myeloid leukemia.

ABSTRACT:

Purpose

In acute myeloid leukemia (AML), initial treatment response by morphologic analysis of bone marrow predicts long-term outcome. Response can now be assessed by minimal residual disease (MRD) monitoring with flow cytometry or polymerase chain reaction (PCR). We determined the relation among the results of these approaches and their prognostic value.

Patients and methods

In the multicenter AML02 study, follow-up bone marrow samples from 203 children and adolescents with newly diagnosed AML were examined by flow cytometry (n = 1,514), morphology (n = 1,382), and PCR amplification of fusion transcripts (n = 508). Results were correlated with treatment outcome.

Results

Among 1,215 samples with less than 5% leukemic myeloblasts by morphology, 100 (8.2%) were MRD posi

SUBMITTER: Inaba H 

PROVIDER: S-EPMC3462046 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature